Advanced bioprocess monitoring by implementation of Proton Transfer Reaction - Mass Spectrometry (PTR-MS) for measurement of volatile components in the bioreactor

To demonstrate the proper functionality of the inlet system, three identical E. coli fermentations were conducted. The reproducibility of these experiments is shown by comparison of a set of process variables (Table 1, Figure 2). Figure 1: Simplified schematic of the setup. Fermenter off-gas is sampled and analyzed continuously. The inlet box, containing the flowcontrollers, is thermally controlled and allows for dilution and calibration and, if needed, multiplexing to analyze several fermenters (not shown).